FIGURE

Fig. 3

ID

ZDB-FIG-150716-6

Publication

Palardy et al., 2015 - Identification of the Mind Bomb1 Interaction Domain in Zebrafish DeltaD

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Fig. 3

Endocytosis of DeltaD deletion mutants.

(A,E,I,M,Q,U) Distribution of zdd2 (green) in COS7 cells transfected with DeltaD (A) or DeltaD ΔA, ΔB, ΔC, ΔD or ΔA-D deletion mutants (E, I, M, Q, U). Surface DeltaD was first labelled by incubation with zdd2 at 4°C for 30′ then, following washout of unbound zdd2, internalization was allowed for 30′ at 37°C. Nuclei were labelled with DAPI (blue). (B-D, F-H, J-L, N-P, R-T, V-X) Distribution of zdd2 (green) in COS7 cells co-transfected with DeltaD constructs and Mib1 (red) following internalization as described above. Each set of 3 panels, respectively, shows distribution of the DeltaD construct (green), Myc-Mib1 (red)/nuclei (blue), and the merged image. See materials and methods for details.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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