FIGURE

Fig. 3

ID

ZDB-FIG-150528-3

Publication

Liu et al., 2015 - DNA methyltransferase 1 functions through C/ebpa to maintain hematopoietic stem and progenitor cells in zebrafish

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Fig. 3

Dnmt1 mRNA rescue and anti-phosphorylated histone H3 (pH3) immunostaining assays in dnmt1-deficient embryos. (A-H) Dnmt1 mRNA rescue assay at 36 hpf (A-D) and 5 dpf (E-H). Dnmt1 ATG morpholino (4 ng) and splicing-specific morpholino (8 ng) effectively reproduced decreased cmyb expression phenotype in wild-type embryos (B, C, F, G). Dnmt1 mRNA rescued the defects of dnmt1 morphants. (I-N) Rescue assay of wild-type dnmt1 mRNA in ldd794 dnmt1 mutants at 36 hpf (I-K) and 3 dpf (L-N). Wild-type dnmt1 mRNA effectively restored cmyb expression in ldd794 dnmt1 mutants at 36 hpf (K) and 3 dpf (N). (A′-N′) Magnified images of the boxed regions in A to N, respectively. Red arrows indicate cmyb-positive HSPCs in the AGM or CHT. (O-T) The proliferation of HSPCs was reduced in dnmt1/ mutants. Double immunostaining of cmyb-EGFP (O-P) and anti-PH3 (Q-R) in the CHT of Tg(cmyb-EGFP) line at 72 hpf. The bottom panel shows merged images (S, T). (U) Statistics of PH3 and cmyb-EGFP double positive cells.

Expression Data

Genes:	EGFP myb
Fish:	TU WT dnmt1^rj794/rj794 (TU) WT + MO4-dnmt1 WT + MO7-dnmt1 zf169Tg dnmt1^rj794/rj794; zf169Tg (all 7)
Knockdown Reagents:	MO4-dnmt1 MO7-dnmt1
Anatomical Terms:	caudal hematopoietic tissue hematopoietic stem cell
Stage Range:	Prim-25 to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	dnmt1^rj794/rj794 (TU) WT + MO4-dnmt1 WT + MO7-dnmt1 dnmt1^rj794/rj794; zf169Tg
Knockdown Reagents:	MO4-dnmt1 MO7-dnmt1
Observed In:	caudal hematopoietic tissue definitive hemopoiesis hematopoietic stem cell
Stage Range:	Prim-25 to Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Hematol. Oncol.