FIGURE

Fig. 6

ID

ZDB-FIG-150406-31

Publication

Boer et al., 2015 - Fascin1-Dependent Filopodia are Required for Directional Migration of a Subset of Neural Crest Cells

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

Depletion of fscn1a-independent NC cell filopodia by low concentration Latrunculin B treatment does not enhance the severity of fscn1a MZ craniofacial defects.

(A) Lateral views of leading edge of cranial NC stream 1 in 26 hpf DMSO or Lat. B-treated Tg(sox10:rfpmb) or Tg(sox10:rfpmb); fscn1a MZ embryos. Scale bar = 25 µm. (B) Quantitation of filopodia number and filopodia length in DMSO or Lat. B-treated Tg(sox10:rfpmb) and Tg(sox10:rfpmb); fscn1a MZ embryos (n = 9 Tg(sox10:rfpmb) + DMSO, n = 10 Tg(sox10:rfpmb) + Lat. B, n = 10 Tg(sox10:rfpmb); fscn1a MZ + DMSO, n = 10 Tg(sox10:rfpmb); fscn1a MZ + Lat. B, *p = 0.02, **p<0.005, ***p<0.001, ****p<0.0001, ns = not significant). (C) Representative ventral views of 5 dpf DMSO or Lat. B-treated wild type (wt) and fscn1a MZ embryos stained with Alcian blue. (D) Quantitation of percentage of embryos with normal or abnormal craniofacial development at 5 dpf. Numbers above columns indicate number of embryos included in analysis (n = 83 wt + DMSO, 93 wt + Lat. B, n = 86 fscn1a MZ + DMSO, n = 72 fscn1a MZ + Lat. B, *p = 0.02).

Expression Data

Gene:	RFP
Fish:	vu234Tg fscn1a^{zd1012/zd1012}; vu234Tg
Condition:	chemical treatment: pharmaceutical
Anatomical Terms:	neural crest filopodium membrane neural crest membrane
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	fscn1a^{zd1011/zd1011} vu234Tg fscn1a^{zd1012/zd1012}; vu234Tg
Condition:	chemical treatment: pharmaceutical
Observed In:	cranial neural crest filopodium Meckel's cartilage palatoquadrate cartilage pharyngeal arch 1 splanchnocranium
Stage Range:	Prim-5 to Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.