Fig. 5

Hh-PKA pathway is required for the development of gnrh3 neurons.

All embryos are at 2A–D, gnrh3 neurons were detected by in situ hybridization with the gnrh3 probe in the wildtype (wt), but not in oep, cyc and smu mutants. E–F. Ectopic GFP expression near the olfactory region in embryos injected with (F) shhb, but not (E) shha mRNA. G–H, gnrh3 signals were normal in control (G) tomatide-treated embryos, but were lost in embryos treated with (H) Hh inhibitor cyclopamine (CYA, 100 μM) at 6–10 hpf. I–K, More ectopic gnrh3-expressing cells were detected after co-injection of gsk3b-MO or cnsk1a-MO with PKI mRNA. L, Gsk3b and csnk1a double MO treatment did not increase the number of ectopic gnrh3-expressing cells. The anterior is to the left in all panels.