FIGURE

Fig. 6

ID

ZDB-FIG-141001-9

Publication

Shi et al., 2014 - Acetylcholine serves as a derepressor in Loperamide-induced Opioid-Induced Bowel Dysfunction (OIBD) in zebrafish

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Fig. 6

(a) The larval fish do not show an obvious developmental defect when treated by ACh-Cl and LH+ACh-Cl for 12 hours compared with control. Left panels are in the BF channel, whereas right are in the GFP channel. (b–c) Quantification data indicate that the effect of exogenous ACh-Cl on gut peristalsis at 4 dpf (b) and 6 dpf (c) after transit incubation for 10–30 minutes. 10, 100, 1000, 2500 and 5000 mg/L ACh-Cl had no influence on the gut movement frequency at 4 dpf (b), whereas 2500 mg/L promote the movement ability obviously at 6 dpf (c). (d) Quantification data show that treatment of exogenous ACh-Cl for 10–30 minutes partially rescue the inhibition phenotype of gut peristalsis caused by LH. (e) Quantification data show that acetylcholinesterase (AChE) inhibits the recovered phenotype by exogenous ACh-Cl. P value is statistical with corresponding control.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT
Conditions:	chemical treatment: acetylcholine chloride chemical treatment: loperamide hydrochloride
Observed In:	gut peristalsis
Stage:	Day 6

Phenotype Detail

Acknowledgments

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