FIGURE

Fig. 5

ID
ZDB-FIG-140923-13
Publication
Diaz-Horta et al., 2014 - FAM65B is a membrane-associated protein of hair cell stereocilia required for hearing
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Fig. 5

Zebrafish model of fam65b sensorineural hearing loss. (A and B) In situ hybridization whole mounts of 3 days postfertilization (dpf), wild-type AB zebrafish with fam65b antisense probe (A) and sense probe (B). The arrow in A indicates fam65b expression in the otic vesicle. (C) (Left) A schematic drawing of morpholinos (red and blue lines) and primer binding sites (red and blue arrows) used for detection of intron 2 and 3 retention, respectively. (Center and Right) RT-PCR signals indicating intron retention in MO1 and MO2 morphants but not in control morphants (COMOs). (DF) Confocal images of saccular hair cells (green) of a control morphant (D) and MO1 (E) and MO2 (F) morphants. (Scale bars, 20 μm.) (G) Comparison of numbers of saccular hair cells (SHC) among controls (n = 9) and MO1 (n = 7) and MO2 (n = 7) morphants. (H) Comparison of numbers of neuromasts per side among controls (n = 10) and MO1 (n = 8) and MO2 (n = 17) morphants. (I) Microphonic potential waveforms of control (Upper trace) and MO1 and MO2 morphants (two Middle traces) in response to 200-Hz sinusoidal stimuli (Lower trace) at 5.8-μm displacement. (J) Comparison of microphonic amplitude (root mean square) among controls (n = 7) and MO1 (n = 7) and MO2 (n = 8) morphants. Stimuli: 200 Hz at 5.8-μm displacement. (K) Comparison of microphonic thresholds among controls (n = 7) and MO1 (n = 7) and MO2 (n = 8) morphants. ANOVAs followed by Tukey posttests were performed to determine the significance of differences. Data are represented as means ± SEM. **P < 0.001, *P < 0.005.

Expression Data
Gene:
Fish:
Anatomical Term:
Stage: Protruding-mouth

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA