|ZFIN ID: ZDB-FIG-140822-2|
Gata3 Expression in Radial Glia and Newborn Neurons Is Injury Induced
(A) Injury paradigm in adult zebrafish telencephalon. DM, dorsomedial; DL, dorsolateral; V, ventral. Red circle is the injury site.
(B) gata3 in situ hybridization (ISH) in uninjured adult telencephalon.
(C) gata3 expression in the lesioned hemisphere after the stab lesion (*) at 3 dpl.
(D) Gata3 immunohistochemistry (IHC) in unlesioned telencephalon.
(E) Gata3 IHC on lesioned telencephalons. (E1–E4) High-magnification images from different regions.
(F) Gata3-positive cells in the ventricular region colocalize with her4.1:GFP (white arrows). Gata3 is also present in GFP-negative cells in close proximity to the GFP-positive ventricular region (yellow arrows). (F1–F3) Single fluorescence channel of the frame in (F).
(G) Time course quantification graph for her4-positive radial glial cells (RGC) in regard to gata3 expression and proliferation. Nonproliferating gata3-positive RGCs, white; proliferating gata3-positive RGCs, yellow; proliferating RGCs that do not express gata3, orange.
(H) Experimental scheme for BrdU treatments.
(I) Gata3, huC:GFP, BrdU triple IHC. Triple-positive cells, white arrows. BrdU-Gata3 double-positive cells lacking huC:GFP in the ventricular region, yellow arrows.
(J) Graph for the ratios for BrdU-Gata3 double-positive and only BrdU-positive cells. Dashed lines indicate the boundary between huC:GFP-negative and positive zones (I–I3).
Scale bars: 100 μm (B–E); 50 μm (E1–E4); 20 μm (F and I). n = 8 fish for (B–E4); 4 for (F), 4 for (G), and 5 for (H and J). Data are represented as mean ± SEM. See also Figure S1.
|EGFP||knu3Tg||physical alteration: anatomical structure||Adult||IHC|
|y83Tg||physical alteration: anatomical structure||Adult||IHC|
|WT||physical alteration: anatomical structure||Adult||ISH|
This image is the copyrighted work of the attributed author or publisher, and
ZFIN has permission only to display this image to its users.
Additional permissions should be obtained from the applicable author or publisher of the image.
Reprinted from Developmental Cell, 23(6), Kizil, C., Kyritsis, N., Dudczig, S., Kroehne, V., Freudenreich, D., Kaslin, J., and Brand, M., Regenerative Neurogenesis from Neural Progenitor Cells Requires Injury-Induced Expression of Gata3, 1230-1237, Copyright (2012) with permission from Elsevier. Full text @ Dev. Cell