FIGURE

Fig. 1

ID

ZDB-FIG-140811-18

Publication

Ju et al., 2014 - Activation of Sonic hedgehog signaling in neural progenitor cells promotes glioma development in the zebrafish optic pathway

Other Figures

All Figure Page

Back to All Figure Page

Fig. 1

GFP expression patterns in the Tg(krt5:EGFP) line. (a) Skin epithelia expression in a 24h.p.f. embryo (side view, anterior to the left). (b) Confocal imaging of GFP-positive cells in the brain of a 72h.p.f. larva (dorsal view, anterior left). (c) Saggital cryosection of an adult fish brain showing GFP expression in the ventricular zones, the OT and the hindbrain (HB). (d) Zoomed-in view of the white frame in (c) showing GFP-positive cells in the ventricular zones resembling radial glia cells partially positive for GFAP (red). (e) GFP expression in the ONH showing little overlap with the retinal Müller glial marker GFAP (red). (f) GFP expression in the reticular astrocytes of the ON. (g) Transverse section through the brain region of a wild-type (WT) 72h.p.f. larva showing GFP expression in the skin (arrow), brain and retina (arrowheads). (h) GFP expression in the brain and the retina was absent in the mindbomb (mb) mutant background, whereas expression in the skin was not affected. All crysections were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) to label nuclei (blue).

Expression Data

Gene:	EGFP
Antibody:	Ab2-gfap
Fish:	sj5Tg mib1^{hi904Tg/hi904Tg}; sj5Tg
Anatomical Terms:	brain cranial nerve II cranial nerve II astrocyte hindbrain integument Muller cell optic nerve head optic tectum radial glial cell retina ventricular zone
Stage Range:	Prim-5 to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Oncogenesis