FIGURE

Fig. 4

ID
ZDB-FIG-140512-47
Publication
Dreosti et al., 2014 - Left-right asymmetry is required for the habenulae to respond to both visual and olfactory stimuli
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Fig. 4

Asymmetric Responses of left dHb Neurons to Light Are Dependent upon the Eyes

(A and B) Dorsal views of live wild-type (A) and chkne2611 mutant (B) fish at 4 dpf.

(C) Dorsal view of the dHb nuclei in a chkne2611 mutant showing that mitral cells inputs remain asymmetrically directed to the right dHb.

(D) Dorsal views of the epithalamus of a Tg(elavl3:GCaMP5G) 3 dpf fish prior (left) and 4 dpf fish subsequent (right) to ablation of the parapineal (pseudocolored blue).

(E–G) Images of color-coded dHb neuron responses to light (red) and odor (blue) in single z plane of a 4 dpf Tg(foxD3:GFP × lhx2a:Gap43-YFP) chkne2611 mutant fish (E), a fish in which eyes were surgically removed at 1 dpf (F), and a Tg(foxD3:GFP × elavl3:GCaMP5G) embryo with late ablation of the parapineal (G).

(H–J) Bar graphs showing average dHb neuron responses to light and odor for chk mutant (H) eye-ablated (I) and parapineal-ablated (J) fish. Fish without eyes show loss of dHb visual responses, whereas fish without a parapineal still show visual responses. For chkne2611 mutant and eye-ablated fish, an average of 12% (n = 6 fish) and 16% (n = 6 fish), respectively, of neurons responded to odor. For late parapineal-ablated fish (n = 3 fish), dHb neurons show an asymmetric distribution of responses to light (9% of neurons) and odor (11% of neurons) comparable with that of the wild-type. Error bars indicate the SEM.

Scale bars, 20 μm. Hb, habenulae; Pp, parapineal; LHb, left habenula nucleus; R Hb, right habenula nucleus.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Curr. Biol.