ZFIN ID: ZDB-FIG-140414-21
Johnson et al., 2014 - Kif11 dependent cell cycle progression in radial glial cells is required for proper neurogenesis in the zebrafish neural tube. Developmental Biology   387(1):73-92 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Terms:
Stage Range: 14-19 somites to High-pec
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: High-pec

Fig. 9 Kif11 is required for secondary motorneurons but not primary motorneurons. (A–F) Visualization of primary motorneuron development in somite 11 over time using the Tg(mnx1:GFP) transgenic line. No difference in the formation of primary motorneurons was observed in STLC treated Tg(mnx1:GFP) transgenic embryos (D–F) as compared to control DMSO treated embryos (A–C). (G,H) Lateral view of the neural tube of Tg(gata2:EGFP) transgenic embryos at 48 hpf to assess secondary motorneurons in control DMSO treated (G) or STLC treated transgenic embryos from 5 to 48 hpf (H). (I) A significant decrease in GFP+ neurons was seen in STLC treated Tg(gata2:EGFP) embryos. Error bars delineate the standard error of the mean. Asterisks indicate a statistical significance (t-test, two tailed, assuming equal variances, p<0.05).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP ml2Tg control 14-19 somites primary motor neuron IFL
Prim-5 primary motor neuron IFL
Prim-15 primary motor neuron IFL
ml2Tg chemical treatment: pharmaceutical 14-19 somites primary motor neuron IFL
Prim-5 primary motor neuron IFL
Prim-15 primary motor neuron IFL
zf35Tg control High-pec secondary motor neuron IFL
zf35Tg chemical treatment: pharmaceutical High-pec secondary motor neuron IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
zf35Tg chemical treatment: pharmaceutical High-pec secondary motor neuron decreased amount, abnormal
Acknowledgments:
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Reprinted from Developmental Biology, 387(1), Johnson, K., Moriarty, C., Tania, N., Ortman, A., Dipietrantonio, K., Edens, B., Eisenman, J., Ok, D., Krikorian, S., Barragan, J., Golé, C., and Barresi, M.J., Kif11 dependent cell cycle progression in radial glial cells is required for proper neurogenesis in the zebrafish neural tube, 73-92, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.