FIGURE

Fig. 5

ID
ZDB-FIG-140311-17
Publication
McCarroll et al., 2013 - Fgf3 and Fgf10a work in concert to promote maturation of the epibranchial placodes in zebrafish
Other Figures
All Figure Page
Back to All Figure Page
Fig. 5

Fgf3 and Fgf10a are required for placode maturation and neurogenesis.

(A-F) Uninjected and fgf10a-MO injected progeny from fgf3+/-;TgBAC(neurog1-DSRed) crosses were collected at 36 hpf and immunolabeled for Pax2a and DSRed to visualize EB placodes and migrating neuroblasts, respectively. Wild-type (A) and fgf3+/- (B) panels shows neurog1:DSRed+ cells undergoing neurogenesis (magenta) at the dorsal aspect of the mature Pax2a+ EB placodes (green; dotted line). fgf3-/- embryos show a loss of properly formed Pax2a+ EB placodes in the region of the prospective glossopharyngeal and three small vagal ganglia and a concurrent loss of neurog1:DSRed+ cells in this region (C; bracket); however, the facial and large vagal placode/ganglia are still present (arrowheads). Analysis of fgf10a-MO injected wild-type (D) and fgf3+/- (E) embryos reveal ectopic neurogenesis as marked by neurog1:DSRed+ cells ventral to the posterior most aspect of the vagal placode (arrow). fgf3-/-;fgf10-MO embryos show a complete loss Pax2a expression in EB placodes and an absence of neurogenesis, except for a few neurog1:DSRed+ cells near the region of the large vagal ganglia (F; asterisk). Abbreviation: ngn:dsred, TgBAC(neurog1-DSRed); f, facial placode; g, glossopharyngeal placode; v, vagal placodes. Scale bar: 50 μm.

Expression Data
Genes:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-25

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-25

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One