FIGURE

Fig. 6

ID

ZDB-FIG-131211-25

Publication

Colombo et al., 2013 - Daam1a mediates asymmetric habenular morphogenesis by regulating dendritic and axonal outgrowth

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Fig. 6

Daam1a cooperates with Ulk2 in habenular neuropil formation. (A-I) The organisation of habenular neuropil at 4.5 hpf as revealed by anti acetylated α-tubulin immunostaining, after injection at the one- to four-cell stage with Co-MO (A), optimal doses of either ulk2^spl-MO (B, 2 ng) or daam1a-MO (C, 8 ng), suboptimal doses of either ulk2^spl-MO (D, 1 ng) or daam1a-MO (E, 4 ng), a combination of suboptimal doses of ulk2^spl-MO and daam1a-MO (F), optimal doses of ulk2^spl-MO (2 ng) combined with either dUlk2 (G) or hDAAM1 (H) mRNAs, or optimal dose of daam1a-MO (8 ng) combined with ulk2 mRNA (I). Images correspond to dorsal views of maximum intensity confocal z-stack projections, with anterior to the top. LHb, left Hb; RHb, right Hb. Scale bar: 20 μm.

Expression Data

Expression Detail

No data available

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-daam1a WT + MO2-ulk2 WT + MO2-daam1a + MO2-ulk2
Knockdown Reagents:	MO2-daam1a MO2-ulk2
Observed In:	dendrite morphogenesis habenula dendrite habenula development
Stage:	Day 4

Phenotype Detail

Acknowledgments

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