FIGURE

Fig. S4

ID

ZDB-FIG-131029-55

Publication

Fang et al., 2013 - Translational profiling of cardiomyocytes identifies an early Jak1/Stat3 injury response required for zebrafish heart regeneration

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Fig. S4

Inhibition of Stat3 in cardiomyocytes has no detectable effects during rapid animal growth. (A) Induction of dnStat3-GFP fluorescence in cardiomyocytes after 4-HT incubation in cmlc2:CreER;β-act2:RSdS or control β-act2:RSdS fish, during slow (normal) and accelerated growth conditions. (B) Control (β-act2:RSdS) and cmlc2:CreER;β-act2:RSdS animals each approximately doubled their mass after 10 d of rapid growth conditions. Data are mean ± SEM n = 12. (C) Representative images of cardiomyocyte proliferation using antibodies against PCNA and Mef2, indicating no detectable difference between control (β-act2:RSdS) and dnStat3-expressing fish (cmlc2:CreER; β-act2:RSdS) after 10 d of either slow or rapid growth. Quantified data are in Fig. 3D. (D) Control (β-act2:RSdS) and cmlc2:CreER;β-act2:RSdS embryos were incubated in 4-HT at 4 d postfertilization and allowed to grow to adulthood. Approximately half of the surviving animals from a clutch of 43 fish were cmlc2:CreER; β-act2:RSdS, indicated by myocardial dnStat3-GFP fluorescence. (Scale bars, 50 μm.)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA