FIGURE

Fig. 5

ID

ZDB-FIG-131015-24

Publication

Wong et al., 2013 - Dorsomorphin promotes survival and germline competence of zebrafish spermatogonial stem cells in culture

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Fig. 5

Dorsomorphin inhibits the growth of FGSCs in 3 week cultures.

(A) Addition of dorsomorphin significantly inhibits FGSC (A) colony formation and (B) cell proliferation in culture. Photomicrographs showing (C) a 3-week old FGSCs (arrows) cultured in the presence of 2 μM dorsomorphin and (D) a 3-week-old FGSC colony cultured in the absence of dorsomorphin. (E) RT-PCR analysis of RNA isolated from a 3-week FGSC culture grown in the presence or absence of dorsomorphin, whole ovaries and feeder cells alone. Dorsomorphin severely reduced the expression of germ cell specific marker genes, dazl, dnd, nanos3, vasa and piwil1. * indicates a significant difference by Student t-tests. Scale bar = 20 μm.

Expression Data

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Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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