FIGURE
Fig. 3
- ID
- ZDB-FIG-130905-3
- Publication
- Mosimann et al., 2013 - Site-directed zebrafish transgenesis into single landing sites with the phiC31 integrase system
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Fig. 3
Non-targeted integration detection. A: Schematic depicting how non-targeted, phiC-31 integrase-independent background insertions of the attB transgene plasmid can be recognized. Such events result in attP, cmlc2:EGFP-negative but attB_ubi:EGFP-positive embryos in the F1, referred to as non-targeted background integrations. B: Example of a recovered clutch with non-targeted integrations. All recovered events of this class stemmed from transgene vector breakage independent of phiC31 and outside of the attB cassette. See text for details; asterisks indicate cmlc2:EGFP-positive hearts. |
Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
Phenotype Detail
Acknowledgments
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