FIGURE

Fig. 4

ID

ZDB-FIG-130826-57

Publication

Pascoal et al., 2013 - Notch signalling is required for the formation of structurally stable muscle fibres in zebrafish

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Fig. 4

Notch signalling is crucial for skeletal muscle integrity and stress fibres formation.

Immunostaining of 5 dpf pectoral fins (A–G′′) with DAPI and phalloidin to label nuclei (blue) and filamentous actin (green), respectively. Stress fibres in the endoskeletal disc cells and intact skeletal muscle fibres are formed in the pectoral fins of siblings (n = 20) (A–A′′) and in DMSO-treated embryos (n = 17) (F-F′′). In the pectoral fins were Notch signalling was disrupted like in mib^ta52b (n = 22) (B–B′′) and mib^m178 (n = 10) (C–C′′) mutants, jagged2 (n = 11) (D–D′′) and Su(H)1+2 (n = 10) (E–E′′) morphants and in DAPT-treated embryos (n = 16) (G–G′′) the endoskeletal disc cells present high levels of actin at the periphery and the skeletal muscle fibres are wavy with gaps between them.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mib1^m178/m178 mib1^ta52b/ta52b WT + MO1-jag2b WT + MO5-rbpja,rbpjb
Knockdown Reagents:	MO1-jag2b MO5-rbpja,rbpjb
Observed In:	pectoral fin actin filament pectoral fin musculature contractile muscle fiber
Stage:	Day 5

Phenotype Detail

Acknowledgments

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