FIGURE

Fig. S4

ID

ZDB-FIG-130802-36

Publication

Choudhuri et al., 2013 - Translation initiation factor eIF3h targets specific transcripts to polysomes during embryogenesis

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Fig. S4

The approach used to compare the synthesis of Crygm2d7 protein from the corresponding FLAG-tagged crygm2d7 mRNA in stage-matched control and eif3ha morphant embryos. (A) Strategy used to generate mRNA by in vitro transcription encoding C-terminally Flag-tagged Crygm2d7. In an alternate construct, the FLAG tag was placed at the N terminus of the protein. (B) The purified RNA was injected along with either a control or eif3ha-specific MO, followed by Western blotting experiments of the embryo-derived lysates ~24 h later, using anti-FLAG antibody.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA