FIGURE

Fig. 4

ID
ZDB-FIG-130618-44
Publication
Yao et al., 2013 - Anaplastic lymphoma kinase is required for neurogenesis in the developing central nervous system of zebrafish
Other Figures
All Figure Page
Back to All Figure Page
Fig. 4

Knock-down of alk does not affect proliferation but induces apoptosis in the hindbrain.

(A–C) Confocal sections of 22 hpf embryos after immunostaining with pH 3 (green) and TUNEL (red). Compared to WT (A), both alk ATG-MO injected (B) or alk Spl-MO injected (C) embryos show pH 3 positive cells at normal positions, but more TUNEL positive cells are evident. Note that the size of neural tube in (B,C) is smaller than in (A). (D) Y-axis indicates numbers of pH 3 positive cells in a 50 µm thick confocal stack of hindbrain. Numbers are not significantly different in alk ATG-MO or alk Spl-MO from WT. (E) Y-axis indicates numbers of TUNEL positive cells in the same samples. Both alk ATG-MO and alk Spl-MO numbers were different from that in WT with high significance (***p<0.001). Mean ± SEM, n = 5 embryos in each group. Unpaired two tailed t-test. Scale bars: 50 μm.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One