FIGURE

Fig. 1

ID

ZDB-FIG-130618-21

Publication

Wong et al., 2013 - Production of zebrafish offspring from cultured female germline stem cells

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Fig. 1

Production of Tg(ziwi:neo) and Tg(ziwi:DsRed) transgenic fish.

(A,B) Diagram of the constructs used to produce the Tg(ziwi:neo) and Tg(ziwi:DsRed) transgenic fish. A 4.8 kb fragment of the ziwi promoter [13] was used to drive expression of (A) neo and (B) DsRed. (C) Neo (green) and (D) Vasa (red) are expressed in the same ovarian germ cells of Tg(ziwi:neo). Higher magnification showing expression of (E) Neo (green) and (F) Vasa (red) in the oogonia (white arrows) of Tg(ziwi:neo); (G) merged photo of E and F. (H) DsRed was detected in the ovarian germ cells of Tg(ziwi:DsRed). Scale bar = 50 μm for C, D, H and 20 μm for E, F, G.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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