Fig. 3

Effects of ccm2l disruption on embryonic vasculature. (A and B) At 52 hpf, control embryos have an appropriately constricted endocardium, while ccm2l morphants exhibit endocardial dilation. (Arrows show lumen diameter). (C and D) Morphology of the CCV at 52 hpf is generally normal in ccm2l morphants. (E and F) At 58 hpf, ISVs are mostly insensitive to ccm2l loss-of-function, although some affected embryos have ISVs that are not fully dilated (arrowhead). (G and H) At 72 hpf, SIVs in affected embryos are frequently mispatterned. (I–K) At 52 hpf, MsVs are approximately the same width in control embryos and affected embryos (arrowheads). A and B are 20× magnification; C–J are 10× magnification. A–F are taken from a lateral perspective with anterior to the left; G and H are dorsal images with anterior to the right. I and J are taken from a dorsal perspective with the anterior end tilted upward. In K, error bars represent standard error. Cont, standard control morpholino.