FIGURE

Fig. S13

ID

ZDB-FIG-130429-29

Publication

Wolf et al., 2013 - Specification of posterior hypothalamic neurons requires coordinated activities of Fezf2, Otp, Sim1a and Foxb1.2

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Fig. S13

The expression of foxb1.2, otp, fezf2, sim1a and wnt8b in the MA. Two-color FISH of 2-dpf wild-type embryos to detect the expression of otpb, sim1a, fezf2, foxb1.2 and wnt8b in the MA in lateral views. (A-A′′) The expression of foxb1.2 partially overlaps with that of fezf2, which extends more posterior and ventral. (B-C′′) By contrast, otpb is expressed dorsally and adjacent to fezf2 expression in the MA (B-B′′), whereas foxb1.2 is more medial to otpb expression (C-C′′). otpb is also expressed in the ventral hypothalamus (asterisk in B), but this expression domain in not included in our summary model for the sake of simplicity. (D-D′′) fezf2 and sim1a partially overlap in the anteriormost part of the dorsal fezf2 expression domain. (E-E′′) foxb1.2 is partially co-expressed with sim1a. (F-J0) otpb and sim1a are mainly co-expressed (F-F′′), whereas wnt8b and foxb1.2, fezf2, otpb or sim1a only partially overlap (G-J′′). Lateral views of 10 μm maximum projections of confocal stacks are shown. PO, preoptic area; HB, hindbrain. Scale bar: 60 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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