FIGURE

Fig. 1

ID
ZDB-FIG-130206-16
Publication
Frömel et al., 2012 - Soluble epoxide hydrolase regulates hematopoietic progenitor cell function via generation of fatty acid diols
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Fig. 1

Role of the sEH in the differentiation of the CVP and the caudal hematopoietic tissue in zebrafish. (A) sEH protein expression and activity in 4-d-old zebrafish embryos treated with control (CTL), sEH-ATG or sEH-Mo13 morpholinos. (B) Confocal images of the CVP in Tg(kdrl:EGFP) zebrafish (48 hpf) treated with control or sEH morpholinos. The anterior part of the embryos is on the left; ca, caudal artery; dlav, dorsal longitudinal anastomotic vessel; se, intersegmental vessel; pav, parachordal vessel. Arrows indicate the delayed secondary sprouting of the parachordal vessels, whereas arrowheads mark missing vessels. (C) Effect of control versus sEH (ATG) morpholinos on the number of cmyb+GFP/lmo2+dsRED cells in the CVP in a 40× field z-stack projection located posterior of the yolk sac extension. (D) 11,12-, 14,15-EET/DHET and 12,13-EpOME/DiHOME levels in extracts from 4-d-old zebrafish treated with solvent (Sol) or the sEH inhibitor t-AUCB (sEH-I). (E) Effect of t-ACUB, 12,13-EpOME, or 12,13-DiHOME on the number of cmyb+GFP/lmo2+dsRED cells in the CVP (four-somite stage until 36 hpf). The graphs summarize data obtained with 5–17 zebrafish per group; **P < 0.01, ***P < 0.001 vs. Sol or CTL, §§P < 0.01 vs. sEH-I alone. (Magnification: B, 100×; E, 400×.)

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA