FIGURE

Fig. 1

ID
ZDB-FIG-121212-13
Publication
Pase et al., 2012 - Neutrophil-Delivered Myeloperoxidase Dampens the Hydrogen Peroxide Burst after Tissue Wounding in Zebrafish
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Fig. 1

Leukocytes Are Required for the Initial Decline in Wound Margin H2O2 Concentrations(A) (i) Mean HyPer ratios reflecting the wound zone [H2O2] profile of WT (injured and uninjured), cloche mutant (clo) and spi1/csf3r morphant embryos. Note the significantly sustained, elevated HyPer ratio/[H2O2] in leukocyte-deficient clo and spi1/csf3r morphants. Insets (ii, iii) show the individual median HyPer data and p values (natural permutation test). The n values are as follows (embryos/independent days/scans): injured WT, 5/2/4; spi1/csf3r morphants, 4/2/3; clo 6/3/5; uninjured WT, 5/1/5.(B) HyPer heat maps depicting [H2O2] across the wounded tail fin at five sequential time points. Accompanying overlaid transmission (Trans) and DsRED2 images demonstrate number and position of Tg(lyz:DsRED2) neutrophils. Note the single DsRED2-positive cell in the spi1/csf3r morphant (black arrow head). clo did not carry the lyz:DsRED2 transgene. Scale bar (applies to all panels) represents 200 μm. Heat map legend applies to all figures. The stills are from Movie S1.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Phenotype Detail
Acknowledgments
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