Fig. 3
- ID
- ZDB-FIG-121130-19
- Publication
- Stulberg et al., 2012 - Crosstalk between Fgf and Wnt signaling in the zebrafish tailbud
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In situ hybridization of dkk1 following LiCl and SU5402 treatment and qPCR validation of selected microarray data. (A) In situ hybridization of dkk1 following LiCl, SU5402, and LiCl/SU5402 treatment. Embryos were stained in parallel for the same duration and images were processed identically. The spatial pattern of dkk1 does not appear to expand, but levels increase following treatment with LiCl, SU5402, and a combination of LiCl and SU5402. Posterior is down. n=4 experiments, 15 embryos per treatment per experiment. (B) We used direct transcriptional targets of the Wnt and Fgf pathways, along with genes involved in somitogenesis, to validate the microarray data. No change in transcription was observed via microarray or qPCR for genes involved in somitogenesis (her1, her7, hes6) following LiCl treatment. Changes identified via microarray in axin2, dkk1, notum1a, and spred3 expression following LiCl treatment were confirmed by qPCR. Inhibition of Fgf signaling using SU5402 resulted in a change in dkk1, notum1a, fgf8, her1, her7, hes6, sef, sprouty4, dusp4, pea3, spred3, tbx6, and tbx24 expression detected via microarray, and these changes were confirmed by qPCR (n = 4). Error bars are SEM. Statistical comparisons were made using the Student′s unpaired t test. *pd0.06, **pd0.01, ***pd0.001. |
Reprinted from Developmental Biology, 369(2), Stulberg, M.J., Lin, A., Zhao, H., and Holley, S.A., Crosstalk between Fgf and Wnt signaling in the zebrafish tailbud, 298-307, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.