ZFIN ID: ZDB-FIG-121130-17
Lotti et al., 2012 - An SMN-Dependent U12 Splicing Event Essential for Motor Circuit Function. Cell   151(2):440-454 Full text @ Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 6 Stasimon Is Required for Motor Neuron Development and Rescues SMN-Dependent Motor Axon Defects in Zebrafish Embryos(A) Representative lateral views of motor axons in Tg(mnx1:GFP) zebrafish embryos expressing GFP in motor neurons and injected with control MO, as well as stas MO, smn MO, and tdp43 MO either with or without coinjected human STAS RNA.(B) Quantification of the effects of Stasimon deficiency on motor axon development in zebrafish. Motor axons were scored in Tg(mnx1:GFP) embryos injected with control MO, stas MO, or stas MO + STAS RNA. Embryos were classified as severe, moderate, mild, or no defects based on the severity of motor axon defects, and the percentage for each group is shown.(C) Quantification of Stasimon effects on SMN-dependent motor axon defects in zebrafish. Motor axons were scored in Tg(mnx1:GFP) embryos injected with control MO, smn MO, or smn MO + STAS RNA and embryos were classified as in (B).(D) Quantification of Stasimon effects on TDP43-dependent motor axon defects in zebrafish. Motor axons were scored in Tg(mnx1:GFP) embryos injected with control MO, tdp43 MO, or tdp43 MO + STAS RNA, and embryos were classified as in (B).Data in all graphs are represented as mean and SEM. See also Figure S6.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP os26Tg control Prim-5 motor neuron axon IFL
Prim-5 spinal cord motor neuron IFL
os26Tg + MO1-als2b chemical treatment: messenger RNA Prim-5 motor neuron axon IFL
Prim-5 spinal cord motor neuron IFL
os26Tg + MO1-als2b standard conditions Prim-5 motor neuron axon IFL
Prim-5 spinal cord motor neuron IFL
os26Tg + MO1-smn1 chemical treatment: messenger RNA Prim-5 motor neuron axon IFL
Prim-5 spinal cord motor neuron IFL
os26Tg + MO1-smn1 standard conditions Prim-5 motor neuron axon IFL
Prim-5 spinal cord motor neuron IFL
os26Tg + MO1-tmem41b chemical treatment: messenger RNA Prim-5 motor neuron axon IFL
Prim-5 spinal cord motor neuron IFL
os26Tg + MO1-tmem41b standard conditions Prim-5 motor neuron axon IFL
Prim-5 spinal cord motor neuron IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
os26Tg + MO1-als2b chemical treatment: messenger RNA Prim-5 motor neuron axon guidance decreased process quality, abnormal
Prim-5 motor neuron axon mislocalised, abnormal
os26Tg + MO1-als2b standard conditions Prim-5 motor neuron axon guidance decreased process quality, abnormal
Prim-5 motor neuron axon mislocalised, abnormal
os26Tg + MO1-smn1 chemical treatment: messenger RNA Prim-5 motor neuron axon guidance process quality, normal
Prim-5 motor neuron axon position, normal
os26Tg + MO1-smn1 standard conditions Prim-5 motor neuron axon guidance decreased process quality, abnormal
Prim-5 motor neuron axon mislocalised, abnormal
os26Tg + MO1-tmem41b chemical treatment: messenger RNA Prim-5 motor neuron axon guidance process quality, normal
Prim-5 motor neuron axon position, normal
os26Tg + MO1-tmem41b standard conditions Prim-5 motor neuron axon guidance decreased process quality, abnormal
Prim-5 motor neuron axon mislocalised, abnormal
Acknowledgments:
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Reprinted from Cell, 151(2), Lotti, F., Imlach, W.L., Saieva, L., Beck, E.S., Hao le, T., Li, D.K., Jiao, W., Mentis, G.Z., Beattie, C.E., McCabe, B.D., and Pellizzoni, L., An SMN-Dependent U12 Splicing Event Essential for Motor Circuit Function, 440-454, Copyright (2012) with permission from Elsevier. Full text @ Cell