FIGURE

Fig. S4

ID

ZDB-FIG-121102-22

Publication

Matsui et al., 2012 - Celf1 regulation of dmrt2a is required for somite symmetry and left-right patterning during zebrafish development

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Fig. S4

Higher amounts of celf1 mRNA lead to additional defects in zebrafish. (A,C,E) Expression of hgg1 (P, polster), dlx3 (NP, anterior edge of the neural plate) or ntl (NT, notochord) in embryos injected with mRFP (A) or celf1 (C,E) mRNAs (150 pg). Animal pole view. Scale bar: 200 μm. (A′,C′,E′) Dorsal view of the embryo, anterior to the top. Injection of 150 pg celf1 mRNA resulted in the formation of bended (C) or short (E′) notochord. The polster did not reach the anterior edge of the neural plate (bracket in E). Epiboly was incomplete (C′,E′). Arrows in A′, C′ and E′ mark edge of the yolk plug. (B,D,F) Lateral view of embryos injected with mRFP (B) or celf1 (D, F) mRNAs (150 pg) at 30 hpf. Various phenotypes, such as short tails, segmentation defects, small eyes, small heads and less pigmentation, were observed (D,F).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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