Fig. 1

Zebrafish sbds is an essential developmental gene with high-level expression in pancreas and surrounding tissues. (A) Expression of sbds examined by in situ hybridization in wild-type zebrafish embryos at 120 hpf. The boxed pancreatic region is shown at higher magnification to illustrate pancreatic and peri-pancreatic expression (arrowheads) of sbds. (B) Adult zebrafish pancreas (boxed) expresses sbds. (C) Sbds^Spl MO results in durable loss of sbds transcript as assessed by RT-PCR. (D) Survival curve for recipients of Sbds^ATG MO illustrates near complete embryonic lethality by 7 dpf, as compared with mock injected embryos. (E) Ribosome profile of lysate prepared from 48-hpf control and Sbds^ATG MO-injected embryos. (F) Quantification of ribosomal subunit ratios, monosomes and polysomes in control and Sbds^ATG MO-injected embryos. Error bars indicate mean ± s.e.m. i, intestine; L, liver; P, pancreas.