FIGURE

Fig. S2

ID
ZDB-FIG-120815-2
Publication
Liu et al., 2012 - betaPix plays a dual role in cerebral vascular stability and angiogenesis, and interacts with integrin alphavbeta8
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Fig. S2

Git1 mRNA expression pattern and knockdown vascular phenotype

(AC) In situ hybridization with a RNA probe against Git1 reveals ubiquitous staining at 1 day post fertilization (dpf) and 2 dpf. At 3 dpf, staining is mostly restricted to the head, but is still broadly distributed. (DF) Confocal microscopy of wild type head (D) and trunk (F) or Git1i1e2 morphant head (E) or trunk (G) at 3 dpf shows a largely normal vascular pattern in Tg(kdrla:GFP)la116 embryos. As compared to wild type embryos, the head vasculature in the region of the central arteries (CtAs) is underdeveloped (arrow). (H) Amplification of Git1 with primers surrounding the deleted exon shows that Git1 mRNA is absent in Git1i1e2 morphants, while the control gene EF1α amplifies normally. Scale bar in all panels represents 100 μm.

Expression Data
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-5 to Protruding-mouth

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth

Phenotype Detail
Acknowledgments
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Reprinted from Developmental Biology, 363(1), Liu, J., Zeng, L., Kennedy, R.M., Gruenig, N.M., and Childs, S.J., betaPix plays a dual role in cerebral vascular stability and angiogenesis, and interacts with integrin alphavbeta8, 95-105, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.