FIGURE

Fig. 2

ID
ZDB-FIG-120720-83
Publication
Zeng et al., 2012 - The smooth muscle microRNA miR-145 regulates gut epithelial development via a paracrine mechanism
Other Figures
All Figure Page
Back to All Figure Page
Fig. 2

Upregulation of Bmp4 and Shha in the gut of miR-145 morphants. Lateral view of whole-mount in situ expression of bmp4 (A, C) and shha (E, G) shows that both genes have increased expression in miR-145 morphant guts at 96 hpf. Longitudinal sections show that bmp4 (B, D) is expressed in smooth muscle cells (smc; arrowheads) and shha (F, H) is expressed in epithelial cells (ec; arrows). The magnification for B, D, F and H is shown by the scale bar in H. (I) Quantitative PCR shows that knockdown of miR-145 leads to strong up-regulation of bmp4 and shha but not the related gene ihha in miR-145 morphants as compared to control embryos (p<0.01, n=3). (J–K) Increased numbers of pSMAD 1, 5, 8 positive (red; white arrow) smooth muscle cells (green) are found in miR-145 morphants (K) as seen in transverse sections of the gut compared to WT (J), counterstained with DAPI (blue). The scale bar in K represents the magnification for J and K. (L) Graph of the proportion of pSMAD 1, 5, 8 positive SMCs. Significance was determined using the student′s t test (p<0.01)

Expression Data
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Day 4

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 367(2), Zeng, L., and Childs, S.J., The smooth muscle microRNA miR-145 regulates gut epithelial development via a paracrine mechanism, 178-186, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.