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Fig. 4

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ZDB-FIG-120717-14

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Iklé et al., 2012 - Identification and characterization of the zebrafish pharyngeal arch-specific enhancer for the basic helix-loop-helix transcription factor Hand2

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Fig. 4

The minimal hand2 arch-specific enhancer is regulated by endothelin signaling. A. A 280 bp region of sequence contained within the larger zhand2 enhancer shown in Fig. 1C was cloned into p5E-FAbas and recombined with a middle entry clone containing a mCherry fluorescent reporter into the Tol2 destination vector pDestTol2CG2, resulting in hand2-directed mCherrry expression. Plasmid is not drawn to scale. B–E. In Tg(shand2:mCherry) embryos, mCherry fluorescence was observed in the Hand2 domain beginning around 30 hpf (B) and continuing through arch development (C, D). mCherry fluorescence can still be detected in the ventral cartilages as late as 7 dpf (E). F, G. Injecting 5 ng of an edn1 morpholino led to decreased hand2 expression at 48 hpf (G) compared to wild-type expression (F). H, I. mCherry activity was similarly decreased in Tg(shand2:mCherry) embryos following injection of the edn1 morpholino (I) compared to uninjected Tg(shand2:mCherry) embryos (H).

Expression Data

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Antibody Labeling

Phenotype Data

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Acknowledgments

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Reprinted from Developmental Biology, 368(1), Iklé, J.M., Artinger, K.B., and Clouthier, D.E., Identification and characterization of the zebrafish pharyngeal arch-specific enhancer for the basic helix-loop-helix transcription factor Hand2, 118-126, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.