FIGURE

Fig. 9

ID
ZDB-FIG-120228-15
Publication
Otten et al., 2012 - Xirp proteins mark injured skeletal muscle in zebrafish
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Fig. 9

Efficient Morpholino antisense oligonucleotide-mediated knock-down of xirp2a does not affect myofibrillogenesis.

(A) Phenotypically, xirp2a morphants are indistinguishable from WT embryos at 24 hpf. Scale bar: 250 μm. (B) Efficient gene knock-down of xirp2a is assessed by immunohistochemistry at 24 hpf. Green: Xirp2a; red: Actin. Scale bar: 20 μm. (C) Complete loss of Xirp2a does not affect myofibrillogenesis and correct sarcomeric organization of skeletal muscle as determined by immunohistochemistry using an antibody against the A/I junction epitope of Titin and rhodamine phalloidin to label sarcomeric Actin at 24 hpf. Green: Titin; red: Actin. Scale bar: 5 μm.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-5

Phenotype Detail
Acknowledgments
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