Fig. 7

alr overexpression promotes liver growth and rescues alr morphants.

A. Overexpression of both alr and enzyme-inactive mutant alr^C131S can promote liver growth. But the wild type alr is more efficient than alr^C131S mutant. The mean liver sizes (means ± SD) are: WT (1.00±0.28), alr mRNA (1.37±0.23) and alr^C131S mRNA (1.13±0.23). B. Both alr and alr^C131S overexpression can rescue alr morphant and restore liver size. The mean liver sizes (means ± SD) are: WT (1.00±0.25), MO (0.78±0.13), MO+alr mRNA (1.30±0.37) and MO+alr^C131S mRNA (1.14±0.30). The black line in the middle of scattered dots indicates the mean liver size in that group. The brackets on top indicate the respective two samples compared by student′s t-test. n, number of embryo analyzed. *, p<0.05; **, p<0.01. C and D. Overexpression of alr and alr^C131S promote liver growth by promoting hepatocyte proliferation. Hepatocyte proliferation demonstrated by immunofluorescent staining of proliferation marker p-H3 in 48 hpf embryos (red color). The tissue sections were counterstained with DAPI (blue) to label nucleus. I: intestine; L: liver. Dash line circles the boundary of liver. *, p<0.05. Sample number and other details are described in the relevant methods section.