FIGURE

Fig. 7

ID

ZDB-FIG-111221-8

Publication

Carten et al., 2011 - Visualizing digestive organ morphology and function using differential fatty acid metabolism in live zebrafish

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Fig. 7

Fat-free (ffr) larvae display abnormal bile accumulation upon BODIPY-FL C₅ feeding. A. Wildtype siblings (controls) from a cross of ffr +/- larvae (6dpf) exhibit normal hepatic accumulation of BODIPY-FL C₅ in lipid droplets (filled arrowhead) and hepatic ducts (arrow). B. Hepatic ducts in ffr -/- larvae leak or abnormally accumulate BODIPY-FL C₅, consistent with the hepatobiliary structural malformations previously identified in these mutants. C. Analog labeling also reveals ffr -/- hepatocytes are unable to secrete bile as indicated by the appearance of cholestatic livers. D. A higher magnification of C. reveals abnormal accumulation of bile in a single ffr -/- hepatocyte. Scale bars = 10μm. (n = 3 feeds; 10 larvae per feed).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	vps51^{p9emcf/p9emcf}
Observed In:	bile acid secretion hepatic duct hepatocyte liver and biliary system
Stage:	Day 6

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 360(2), Carten, J.D., Bradford, M.K., and Farber, S., Visualizing digestive organ morphology and function using differential fatty acid metabolism in live zebrafish, 276-85, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.