FIGURE

Fig. 4

ID
ZDB-FIG-111220-37
Publication
Chatterjee et al., 2011 - Conserved And Non-Conserved Enhancers Direct Tissue Specific Transcription In Ancient Germ Layer Specific Developmental Control Genes
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Fig. 4

BAC modification process. (A) The UCSC genome browser showing two BACs (red arrows) spanning the gene nkx3.2 in zebrafish. (B) BAC modification by homologous recombination to insert a reporter gene and a drug selection cassette next to the translation start site of the gene (ATG). (C) A zebrafish carrying the modified BAC for the gene nkx3.2 expressing EGFP in sclerotomes (white arrows) at 24 hpf. Abbreviations: EGFP, enhanced green fluorescent protein; NEO, neomycin; LHA, left homology arm; RHA, right homology arm; TGA, stop codon.

Expression Data
Gene:
Fish:
Anatomical Terms:
Stage Range: Shield to Prim-5

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.