FIGURE

Fig. S2

ID
ZDB-FIG-111020-2
Publication
Hinits et al., 2011 - Defective cranial skeletal development, larval lethality and haploinsufficiency in Myod mutant zebrafish
Other Figures
All Figure Page
Back to All Figure Page
Fig. S2

Myf5 or Myod is required for myogenesis. Dorsal flatmounts (A,D—bottom panel), ventral (D—top panel) and lateral (B,C) wholemounts or transverse cryosections (E) of zebrafish embryos from myf5hu2022/+;myodfh261/+ in-cross analysed at the indicated stage by immunohistochemistry for slow MyHC (A) or all MyHC (E) or in situ mRNA hybridization for smyhc1 (B) or mylz2 (C,D), anterior to top (A–D), dorsal to top (E). A. At 15 s, most embryos show strong slow muscle differentiation, but approximately 3/16ths (genotyped as myodfh261/fh261;myf5hu2022/+ or myodfh261/fh261;myf5+/+) have less muscle and 1/16th (genotyped as myodfh261/fh261;myf5hu2022/hu2022) have no detectable muscle. B. mRNAs encoding slow myosin is reduced in putative myodfh261 mutants and absent in doubles. C,D. At 72 hpf, no mylz2 mRNA is detected in genotyped double mutants, in which the trunk and tail are severely reduced in size. E. At 120 hpf, double mutant lacks MyHC in the gut extension region and has a reduced somite area. Note the similar sizes of non-muscle tissues. Bars = 100 μm.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 358(1), Hinits, Y., Williams, V.C., Sweetman, D., Donn, T.M., Ma, T.P., Moens, C.B., and Hughes, S.M., Defective cranial skeletal development, larval lethality and haploinsufficiency in Myod mutant zebrafish, 102-12, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.