FIGURE

Fig. 9

ID
ZDB-FIG-110920-77
Publication
Eames et al., 2011 - Mutations in fam20b and xylt1 Reveal That Cartilage Matrix Controls Timing of Endochondral Ossification by Inhibiting Chondrocyte Maturation
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Fig. 9

Precocious bone formation and osteoblast differentiation in perichondria of fam20b and xylt1 mutants.

A–F, Alcian blue/Alizarin red-stained ceratohyals. G–I, live Alizarin red fluorescence of fam20bb1127;Tg(sp7:EGFP)b1212 and xylt1b1128;Tg(sp7:EGFP)b1212 larvae. J–O, whole-mount in situ hybridization of 4 dpf ceratohyals. No signs of Alizarin red staining were observed at 3 dpf in ceratohyals of wild types (A), or fam20bb1127 (B) or xylt1b1128 (C) mutants. By 4.5 dpf, Alizarin red staining was still absent from ceratohyals of wild-type larvae (D, arrow), although it was detected in ceratohyals of fam20bb1127 (E, arrow) and xylt1b1128 (F, arrow) mutants. Both GFP expression and Alizarin red staining were at background levels in the perichondrium of the ceratohyal in 4.5 dpf wild types (G, arrow), but were obvious in the fam20bb1127 and xylt1b1128 mutant perichondria (H,I, arrow). At 4 dpf, col10a1 expression was not detected in the ceratohyal perichondrium of wild types (J, arrow), but was expressed highly in fam20bb1127 (K, arrow) and xylt1b1128 (L, arrow) mutant perichondria. Abbreviations: bsr = branchiostegal ray.

Expression Data
Genes:
Fish:
Anatomical Terms:
Stage: Day 4

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage Range: Protruding-mouth to Day 4

Phenotype Detail
Acknowledgments
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