FIGURE

Fig. 7

ID
ZDB-FIG-110909-35
Publication
Johnson et al., 2011 - Vgll2a is required for neural crest cell survival during zebrafish craniofacial development
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Fig. 7

Expression of vgll2a is regulated by FGF and RA signaling. Dorsal views of embryos processed by ISH using a vgll2a probe following treatment with DMSO (control, A), SU5402 (B), DEAB (C), and RA (E). Embryos treated with 20 μM SU5402 to inhibit FGF signaling for one hour from 23 to 24 hpf and processed for by ISH for vgll2a expression show a reduction in vgll2a mRNA in the endodermal and ectodermal pharyngeal epithelia surrounding the arches (B, n = 22/23) compared to DMSO-treated control embryos (A, n = 32/34). Embryos treated from 23 to 24 hpf with 100 μM DEAB to inhibit RA synthesis (C, n = 25/27) exhibit increased vgll2a expression in the pharyngeal epithelia while treatment with 100 nM all-trans RA (D, n = 25/25) results in reduced expression of vgll2a in this domain. Other domains of vgll2a expression were not affected in these treatments (data not shown). Anterior is to the left in all panels.

Expression Data
Gene:
Fish:
Conditions:
Anatomical Term:
Stage: Prim-5

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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Reprinted from Developmental Biology, 357(1), Johnson, C.W., Hernandez-Lagunas, L., Feng, W., Melvin, V.S., Williams, T., and Artinger, K.B., Vgll2a is required for neural crest cell survival during zebrafish craniofacial development, 269-81, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.