Fig. 5
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- ZDB-FIG-110907-26
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- Belting et al., 2011 - Pou5f1 contributes to dorsoventral patterning by positive regulation of vox and modulation of fgf8a expression
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vox is a direct transcriptional target of Pou5f1. (A) Whole-mount in situ hybridization of 5 hpf wild-type control embryos and embryos injected with Pou5f1-VP16; probes are as indicated. Embryos were treated with cycloheximide (CHX) starting at the 64-cell stage to prevent translation. Absence of ntl staining served as a control for zygotic translation block. Note that Pou5f1-VP16 induced vent and vox genes. Embryos probed with ntl and vox are shown in lateral view, and embryos probed with bmp2b and vent stained embryos are shown in animal view. (B) Whole-mount in situ hybridization of CHX-treated non-injected MZspg embryos and MZspg embryos injected with pou5f1-VP16 and analyzed with vox or vent probes, animal view (44 out of 48 embryos showed increased vent expression). (C, D) Quantitative PCR: vox mRNA levels were measured in CHX treated and untreated shield stage MZspg embryos, which were non-injected, or injected with mRNAs at one cell stage as indicated. vox expression values were normalized to housekeeping gene ef1alpha expression levels (ddCT method) and then to non-injected control (set as 1). (C) Pou5f1-VP16 induces vox, and Pou5f1-En repressed vox expression in the presence of CHX in MZspg. (D) Pou5f1 induces vox in the presence of CHX in MZspg. |
Reprinted from Developmental Biology, 356(2), Belting, H.G., Wendik, B., Lunde, K., Leichsenring, M., Mössner, R., Driever, W., and Onichtchouk, D., Pou5f1 contributes to dorsoventral patterning by positive regulation of vox and modulation of fgf8a expression, 323-36, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.