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ZDB-FIG-110624-48

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Putiri et al., 2011 - The zebrafish maternal-effect gene mission impossible encodes the DEAH-box helicase Dhx16 and is essential for the expression of downstream endodermal genes

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Fig. s2

Gene expression markers of the hypoblast layer show reduced morphogenetic movements in maternal mis mutant (B,D,F) compared to wild-type (A,C,E). Embryos fixed at 85% epiboly (8.5 h.p.f.) and labeled to detect lefty2 (A,B), cyclops (C,D), and no tail (E,F) mRNA by in situ hybridization. In mutant embryos, the extension of the dorsal expression for these genes (double-headed arrows in A–E, a dorsal domain is not observed in F) is reduced along the anteroposterior axis, consistent with reduced involution of the hypoblast. Where not obscured by marginal expression, the dorsal domain is wider in mutants (brackets in A–D), consistent with defects in dorsal convergence.

Expression Data

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Antibody Labeling

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Reprinted from Developmental Biology, 353(2), Putiri, E., and Pelegri, F., The zebrafish maternal-effect gene mission impossible encodes the DEAH-box helicase Dhx16 and is essential for the expression of downstream endodermal genes, 275-289, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.