Fig. 3

Temporal control of Gal80 expression.

(A) Schematic representation of the construct hsp70:Gal80-myc. (B) Anti-myc western blotting of 48 hpf fish resulting from a cross of Tg[hsp70:Gal80-myc] fish after 0, 30 and 60 minutes of heat-shock. (C) Embryos resulting from a cross between Tg[hsp70:Gal80-Myc] and Tg[UAS:Kaede] fish were not submitted (a–c) or submitted to a one-hour heat-shock (d–f). Representative specimens are depicted for anti-Gal80 in situ hybridization at 30 hpf (a, d) or for Kaede expression at 2 dpf (b–c, d–e). (Scale bars: 150 µm). (D) Embryos resulting from a cross between Tg[hsp70:Gal80-Myc] and Tg[UAS:Kaede] fish were injected with a DNA encoding full-length Gal4 under the control of the β-actin promoter. At 24 hpf, the embryos were subjected to a 30-minute heat-shock at 39 degrees and immediately photo-converted. The following day, immunostaining anti-myc (c, g) was performed on fish expressing Gal80 (a–d) or not expressing Gal80 (e–h). The figure depicts muscle fibers for the expression of Kaede^red (a, e) and Kaede^green (b, f). (Scale bars: 20 μm).