FIGURE

Fig. 2

ID

ZDB-FIG-101210-5

Publication

Sartori da Silva et al., 2010 - Essential Role for the d-Asb11 cul5 Box Domain for Proper Notch Signaling and Neural Cell Fate Decisions In Vivo

Other Figures

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Fig. 2

Phenotypic assays on wild type and asb11^cul embryos.

(A), Morphological analysis of wild type and mutant embryos at 48 and 72hpf. (B), (left) Anterior view of wild type and mutant embryos at 10hpf after whole mount in situ hybridization, WISH, using probe against d-asb11. (right) Graph shows the quantification of the respective expressions using qPCR. (C), (left) Endogenous d-Asb11 in wild type (WT), heterozygous (asb11+/-) and mutant (asb11^cul) embryos at 12 hpf was detected by immunoblotting using anti-d-Asb11 antibody. (right) Graph quantifies 3 individual experiments, with 30 embryos/genotype/experiment.

Expression Data

Gene:	asb11
Fish:	WT asb11^zf210/zf210 asb11^zf210/+
Anatomical Terms:	neural plate polster whole organism
Stage Range:	Bud to 5-9 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	asb11^zf210/zf210
Observed In:	pericardium
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

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