Fig. S1

The phenotypes of Id3 morphants can be rescued by co-injection with Id3 mRNA. A: Phenotypes of Id3 morphants. Morphants show destroyed MHB at 24 hours postfertilization (hpf) and enlarged fourth ventricle at 48 hpf. The severe ones in the right panel show smaller a body. Arrowheads indicate the MHB, while arrows indicate the fourth ventricle. B: The defects caused by the Id3 morpholino can be rescued by ΔId3 mRNA. Arrows indicate the MHB. Quantitative analysis indicates that more than 70% of embryos injected with Id3 morpholino showed abnormal phenotype, whereas only approximately 30% of embryos co-injected with ΔId3 mRNA showed abnormal phenotypes. C: In situ hybridization of the MHB-specific gene Her5 indicates that the MHB is disturbed by Id3 morpholino. MHB, mid–hindbrain boundary.