Global phenotypes arise in sox17 morphants, but not in DFCsox17 MO embryos. A: Location of the sox17 MO. 32-Fluorescein tagged sox17 MO was widely distributed in the embryos injected at the one-cell stage (B: shield; C: late somite stage). D,E: 22hpf-old embryos injected at the one-cell stage with 10 ng control (CO) MO, and sox17 MO, respectively. F-I: Injection into the yolk at the midblastula stage excludes the MO from most embryonic cells except the DFC (F, arrow, 80% epiboly) and a few cells in the tail (G, arrows. 1dpf). H–K: Embryos injected into the yolk at the midblastula stage with 20 ng CO MO (H,J) or sox17 MO (I,K) were similar. Pictures taken under fluorescence illumination at about 8-somite stage (H,I), and in visible light at 2dpf (J,K). L: The efficiency of sox17 MO, 10 ng, injected at the one-cell stage, was assessed at 60-80% epiboly by RT-PCR with primers shown in A; β-act was used as control. All pictures are lateral view, except vegetal view with dorsal on top in F. C-E and H-I: anterior to the left; G: anterior to the right.
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