FIGURE

Fig. 5

ID

ZDB-FIG-100913-8

Publication

May-Simera et al., 2010 - Bbs8, together with the planar cell polarity protein Vangl2, is required to establish left-right asymmetry in zebrafish

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Fig. 5

Positions of the nucleus and basal body in Kupffer′s vesicle cells. A, B, E, F) Measurements of the nearest nuclei to the apical membrane. In tri^-/- embryos, the distance of the nucleus (DAPI) and the apical membrane is increased compared to either bbs8-morphants or sibling controls. C, D) Basal bodies (γ-Tub) and cilia (Ac-Tub) appear to be more detached from the apical membrane of the KV in tri^-/- embryos. G) Table depicting nuclear distance from the apical membrane (μm) in injected and uninjected tri embryos.

Expression Data

Expression Detail

No data available

Antibody Labeling

Phenotype Data

Fish:	vangl2^m209/m209 TL + MO3-ttc8 vangl2^m209/m209 + MO3-ttc8
Knockdown Reagent:	MO3-ttc8
Observed In:	Kupffer's vesicle apical plasma membrane Kupffer's vesicle ciliary basal body Kupffer's vesicle nucleus
Stage:	5-9 somites

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 345(2), May-Simera, H.L., Kai, M., Hernandez, V., Osborn, D.P., Tada, M., and Beales, P.L., Bbs8, together with the planar cell polarity protein Vangl2, is required to establish left-right asymmetry in zebrafish, 215-225, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.