FIGURE

Fig. 4

ID
ZDB-FIG-100616-7
Publication
Landsverk et al., 2010 - Alternative splicing of sept9a and sept9b in zebrafish produces multiple mRNA transcripts expressed throughout development
Other Figures
All Figure Page
Back to All Figure Page
Fig. 4

Characterization of sept9a morphant and overexpression embryos.

Embryos were injected at the one-cell stage with morpholinos targeted to all sept9a transcripts (MO5), sept9a_tv1 only (MO2), mismatch controls (MO5MM, MO2MM), or sept9a_tv1 mRNA with and without MO2. Morphants shown were injected with 2.5 ng morpholino. At 48 hpf, the phenotypes were assessed by morphological criteria, according to severity. A,D: Class I morphants had defects in epidermal integrity and yolk extension and minor curvature of the tail. B,E: Class II morphants had a curved body axis in addition to the defects observed in class I. Class III morphants had a severely shortened body axis (data not shown). Arrows indicate yolk extension defects. All classes exhibited defects in blood circulation. G: Coinjection of 1 pg of sept9a_tv1 mRNA with 5 ng of MO2 partially rescued the observed phenotypes. H,I: Embryos injected with as little as 4 pg of sept9a_tv1 mRNA often had phenotypes similar to those of sept9a morphants including epidermal aggregates (arrow), blood pooling, and tail edema (bracket). (OE) indicates over expression. C,F: Control mismatch morpholinos did not present a phenotype. J: Graphical representation of MO classes at various concentrations. The number of embryos tested in each experiments is indicated by (n) on top of each column. K: sept9a splice morpholinos inhibit sept9a transcript splicing. RT-PCR analysis was performed on 24 hpf wild-type embryos, embryos injected with 2.5 ng MO5 or MO2 (pooled classes I and II), or 5 bp mismatch controls. MO5 embryos show a complete loss of sept9a_tv1 and sept9a_tv7. The presence of a low level of sept9a exons 3–5 transcripts in MO5-injected embryos may be due to maternal mRNA. MO2 embryos show a decrease in sept9a_tv1 compared to wild-type while the other transcripts are not affected.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One