FIGURE

Fig. 4

ID

ZDB-FIG-100520-14

Publication

Branam et al., 2010 - Zebrafish Chordin-like and Chordin Are Functionally Redundant in Regulating Patterning of the Dorsoventral Axis

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

Chl and CHL1 are substrates for BMP1/Tolloid-like proteinases. (A) A Coomassie-stained SDS–PAGE gel is shown of protein molecular weight markers (lane 1) and of ∼ 55-kDa secreted and purified Chl (lane 2). (B–D) Immunoblotting with anti-FLAG antibody (B and D) or anti-CHL1 antibody (C) is shown for in vitro assays in which recombinant Chl was incubated in the absence (-) or presence (+) of Bmp1a (B) or in which recombinant short, neuralin form of CHL1 (C) or CHL2 (D) was incubated in the absence (o/n) or presence of mammalian BMP1, mTLD, mTLL1, or mTLL2. Positions of the ∼ 30-kDa Chl (B) and ∼ 20-kDa and smaller CHL1 (C) cleavage products are shown. In B and D, asterisks mark the positions of FLAG-tagged BMP1 and related proteinases. Molecular masses (in kDa) are indicated for protein standards.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 341(2), Branam, A.M., Hoffman, G.G., Pelegri, F., and Greenspan, D.S., Zebrafish Chordin-like and Chordin Are Functionally Redundant in Regulating Patterning of the Dorsoventral Axis, 444-458, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.