FIGURE

Fig. 5

ID

ZDB-FIG-100504-17

Publication

Tay et al., 2010 - A vertebrate-specific Chp-PAK-PIX pathway maintains E-cadherin at adherens junctions during zebrafish epiboly

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Fig. 5

Localization of β-cat at the AJs requires Chp signaling.

(A) A middle sagittal plane of the embryo at 60% epiboly immunostained with β-cat is also derived from the Z stack. β-cat was no longer maintained at the AJs in the EVL and was found predominately in cytoplasm. Scale bars represent 20 μm. (B) Immunostaining of β-cat comparing the EVL and DEL of un–injected control and Chp morphant. Levels of anti-β-cat staining at AJs were severely reduced in Chp morphants. The delocalized β-cat protein was detected within intracellular structures, but no nuclear enrichment was observed relative to controls.

Expression Data

Antibody:	Ab2-ctnnb
Fish:	WT WT + MO1-rhov WT + MO2-rhov
Knockdown Reagents:	MO1-rhov MO2-rhov
Anatomical Terms:	DEL adherens junction DEL cytoplasm EVL adherens junction EVL cytoplasm
Stage:	Shield

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-rhov WT + MO2-rhov
Knockdown Reagents:	MO1-rhov MO2-rhov
Observed In:	EVL adherens junction protein localization to adherens junction
Stage:	Shield

Phenotype Detail

Acknowledgments

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