ZFIN ID: ZDB-FIG-100408-6
Veldman et al., 2010 - Tuba1a gene expression is regulated by KLF6/7 and is necessary for CNS development and regeneration in zebrafish. Molecular and cellular neurosciences   43(4):370-383 Full text @ Mol. Cell Neurosci.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-15 to Prim-25

Fig. 3 Knockdown of Tuba1a, but not Tuba1b, suppresses CNS formation in developing embryos. Control, tuba1a or tuba1b-targeting morpholinos (MO) were injected into single cell zebrafish embryos. Top graphs show that tuba1a MO-dependent phenotypes at 30 h post fertilization (hpf) are concentration-dependent (tuba1a MO concentrations are 0, 0.065, 0.125 and 0.25 mM; control MO was used to keep total MO concentrations at 0.25 mM). Bottom images show representative pictures of MOinjected (0.125 mM) embryo phenotypes at 30 and 52 hpf. To inhibit apoptosis and examine MO specificity, some experiments included in vitro transcribed bcl2 mRNA (25 pg) or tuba1a mRNA (12.5 pg) that was engineered to escape MO-mediated knockdown. Note that tuba1a, but not tuba1b MO severely disrupted development, especially of anterior structures; however, inclusion of bcl2 or tuba1a mRNA diminished the severity of Tuba1a knockdown. Three different tuba1a mRNA rescue phenotypes are shown at 52 hpf. Similar results were obtained with a second MO targeting different tuba1a and tuba1b sequences.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-tuba1a standard conditions Prim-15 apoptotic process increased occurrence, abnormal
Prim-15 brain development disrupted, abnormal
Prim-15 brain morphology, abnormal
Prim-15 embryo development process quality, abnormal
Prim-15 retina development in camera-type eye disrupted, abnormal
Prim-15 retina morphology, abnormal
Prim-25 whole organism dead, abnormal
Prim-25 whole organism viability, abnormal
WT + MO1-tuba1b standard conditions Prim-15 whole organism morphology, normal
WT + MO2-tuba1a standard conditions Prim-15 apoptotic process increased occurrence, abnormal
Prim-15 brain development disrupted, abnormal
Prim-15 brain morphology, abnormal
Prim-15 embryo development process quality, abnormal
Prim-15 retina development in camera-type eye disrupted, abnormal
Prim-15 retina morphology, abnormal
Prim-25 whole organism dead, abnormal
Prim-25 whole organism viability, abnormal
WT + MO2-tuba1b standard conditions Prim-15 whole organism morphology, normal
Acknowledgments:
ZFIN wishes to thank the journal Molecular and cellular neurosciences for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Molecular and cellular neurosciences, 43(4), Veldman, M.B., Bemben, M.A., and Goldman, D., Tuba1a gene expression is regulated by KLF6/7 and is necessary for CNS development and regeneration in zebrafish, 370-383, Copyright (2010) with permission from Elsevier. Full text @ Mol. Cell Neurosci.