FIGURE

Fig. 2

ID
ZDB-FIG-100304-13
Publication
Wan et al., 2010 - Negative Feedback Regulation of Wnt4 Signaling by EAF1 and EAF2/U19
Other Figures
All Figure Page
Back to All Figure Page
Fig. 2

Zebrafish wnt4a can activate the zebrafish eaf1 and eaf2/U19 promoters.

(A) The sequence of the 1.2 kb zebrafish eaf1 promoter and the positions of primers used for subcloning. The transcriptional initial site is indicated by +1. (B) Zebrafish embryos were co-injected with the eaf1 promoter reporter and either a control vector or a vector expressing Myc-Want4a. The luciferase activity was normalized to Renilla and reported as the mean ± standard deviation (SD). (C) The sequence of the 1.0 kb zebrafish eaf2/u19 promoter and the primers used for subcloning. The transcriptional initial site is indicated by +1. (D) The expression of Myc-Wnt4a was verified by Western blot using an anti-Myc antibody. (E) Zebrafish embryos were co-injected with the eaf2/u19 promoter reporter and either a control vector or a vector expressing Myc-Wnt4a. The luciferase activity was normalized to Renilla and reported as the mean ± SD.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One