FIGURE

Fig. 2

ID

ZDB-FIG-091214-34

Publication

Takeda et al., 2009 - DAZL relieves miRNA-mediated repression of germline mRNAs by controlling poly(A) tail length in zebrafish

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Fig. 2

Identification of the cis-element required for the regulation by DAZL.

(A) Schematic representation of tdrd7 3′UTR mutant constructs. Putative DAZL-binding sites (GUUC; red, GUUA; yellow), miR-430 target sites (blue), and mutated DAZL-binding sites (gray) are indicated. Nucleotide positions relative to the stop codon are shown above. The exogenous miR-430 target site (IPT^miR-430) in the IPT^miR-430 91–123 and IPT^miR-430 91–123 mt constructs were derived from the nanos1 3′UTR [5]. (B) Injection experiments of mutant GFP-tdrd7 mRNA and DsRed mRNA with or without the mRNA encoding Myc-DAZL. GFP and DsRed were analyzed at 24 hpf. Fold change of normalized GFP fluorescence relative to mock control is shown on the right.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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